sulfo-Cyanine5.5 is a water-soluble, hydrophilic far-red fluorophore, an analog of Cy5.5®. Like other cyanine dyes, sulfo-Cyanine5.5 has an outstanding molar extinction coefficient, giving rise to its bright fluorescence. The molecule contains four sulfo groups that provide hydrophilicity and negative charge to the fluorophore and minimize non-specific binding. This reagent contains tetrazine moiety that reacts with trans-cyclooctenes, cyclopropenes, and some strained cyclooctynes to form stable conjugates via an inverted electron demand [4+2]-cycloaddition (IEDDA). The reagent possesses high aqueous solubility and hydrophilicity and is recommended for labeling biomolecules in an aqueous environment.
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