DBCO-N-bis(PEG8-NHS ester) is a trifunctional reagent containing two activated NHS esters and a DBCO moiety. The DBCO is a strained cyclooctyne and reacts spontaneously with azide groups to form stable triazole links with the target molecule. The NHS esters are activated towards attack from nucleophiles such as amines and alcohols to form amides or esters respectively.
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