8-Azido-3,8-dideoxy-D-manno-octulosonic acid (Kdo Azide) is an analogue of the natural 3-deoxy-D-manno-octulosonic acid (Kdo) that contains a very small modification, specifically an azido moiety. This molecule can be incorporated by cultured cells (Gram-negative Bacteria, for example) and incorporated into glycans (LPS, for example) during active glycan biosynthesis. Detection utilizes the chemoselective ligation or "click" reaction between an azide and an alkyne or cyclooctyne.
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